Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, DyLight® 550 conjugated,min, cross-reactivity to bovine, human, or Mouse lgG or serum proteins

(Cat#: AS12 1968)
AS12_1968
AS12_1968
Description
  • Immunogen: Purified rabbit IgG, whole molecule
  • Host: Goat
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified goat IgG.
  • Format: Lyophilized
  • Quantity: 0.5 mg
  • Reconstitution: For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
  • Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
  • Recommended dilutions: 1 : 20-1 : 2000 for most applications
  • Goat anti-rabbit IgG (H&L) - F(ab)'2 fragment, DyLight®550 Conjugate, min. cross-reactivity to bovine, human, or mouse lgG or serum proteins is a secondary antibody conjugated to DyLight® 550, which binds to all rabbit IgG (H&L), F(ab)'2 fragment in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
  • Provost et al. (2019). Sci Rep. 2019 Nov 29;9(1):17967. doi: 10.1038/s41598-019-53528-0.
  • Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1% (w/v) BSA. Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative

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