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{{/_source.additionalInfo}}Anti-ACT | Actin (polyclonal)
(Cat#: AS13 2640)
Description
- Immunogen: ca. 100 amino acids of recombinant actin conserved more than 80% in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47 AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293 AT1G49240 , actin-11 P53496 , AT3G12110 ,actin-12 P53497 AT3G46520
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 50 µl
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: immunofluorescence (IF), Western blot (WB)
- Recommended dilutions: 1-100 - 1 : 250 (IF), 1 : 3000-1 : 5000 (WB)
- Expected | apparent MW: 41.6 | 45 kDa
- Confirmed reactivity: Agostis stolonifera cv. ‘Penncross’,Arabidopsis thaliana, Brassica napus, Cucumis sativus, Cyanthobasis fruticulosa, Cynara cardunculus, Fragaria x ananassa,, Glycine max, Hordeum vulgare, Nicotiana tabacum, Odontarrhena lesbiaca, Petrosimonia nigdeensis, Phaseolus vulgaris, Phaeodactylum tricornutum, Phoenix dactylifera, Picrorhiza kurroa,Salsola grandis, Salsola tragus, Setaria italica, Solanum tuberosum, Triticum aestivum, Vigna unguiculata, Zea mays
- Not reactive in: Chlamydomonas reinhardtii (too high background for this species)
- Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
- Uflewski et al. (2024). The thylakoid proton antiporter KEA3 regulates photosynthesis in response to the chloroplast energy status. Nat Commun. 2024 Mar 30;15(1):2792. doi: 10.1038/s41467-024-47151-5. Lorrai et al. (2024). Cell wall integrity modulates HOOKLESS1 and PHYTOCHROME INTERACTING FACTOR4 expression controlling apical hook formation. Plant Physiol. 2024 Jul 8:kiae370. doi: 10.1093/plphys/kiae370. Salesse-Smith et al. (2024). Greater mesophyll conductance and leaf photosynthesis in the field through modified cell wall porosity and thickness via AtCGR3 expression in tobacco. Plant Biotechnol J. 2024 Apr 30.doi: 10.1111/pbi.14364. Ciesielska et al. (2024). S2P2-the chloroplast-located intramembrane protease and its impact on the stoichiometry and functioning of the photosynthetic apparatus of A. thaliana. Front Plant Sci. 2024 Mar 15:15:1372318. doi: 10.3389/fpls.2024.1372318. Pavlovič et al. (2024). Diethyl ether anaesthesia inhibits de-etiolation of barley seedlings by locking them in intermediate skoto-photomorphogenetic state. Physiol Plantarum, Volume176, Issue 1.Soria et al. (2024).Functional resilience: An active oxidative phosphorylation system prevails amid foreign proteins in holoparasitic plants. Current Plant Biology Volume 37, March 2024, 100322.Blagojevic et al. (2024). Heat stress promotes Arabidopsis AGO1 phase separation and association with stress granule components. iScience. 2024 Feb 6;27(3):109151. doi: 10.1016/j.isci.2024.109151. eCollection 2024 Mar 15. Gong et al. (2024). HYPK controls stability and catalytic activity of the N-terminal acetyltransferase A in Arabidopsis thaliana. Cell Rep. 2024 Feb 15;43(2):113768.doi: 10.1016/j.celrep.2024.113768. Llamas et al. (2023). In planta expression of human polyQ-expanded huntingtin fragment reveals mechanisms to prevent disease-related protein aggregation. Nat Aging. 2023 Nov;3(11):1345-1357.doi: 10.1038/s43587-023-00502-1. Oláh et al. (2023). Suboptimal zinc supply affects the S-nitrosoglutathione reductase enzyme and nitric oxide signaling in Arabidopsis. Plant Stress Volume 10, December 2023, 100250. Ji et al. (2023). Evolution of a plant growth-regulatory protein interaction specificity. Nat Plants. 2023 Dec;9(12):2059-2070.doi: 10.1038/s41477-023-01556-0.
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