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{{/_source.additionalInfo}}Anti-V-ATPase holoenzyme
(Cat#: AS17 4158)
Description
- Immunogen: V-ATPase complex from Avena sativa purified by gel filtration Ward and Sze 1992
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 50 µl
- Reconstitution: For reconstitution add 50 µl, of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 400 - 1: 1000 (WB)
- Expected | apparent MW: 100, 44 , 42, 36 , 16, 13 kDa
- Confirmed reactivity: Avena sativa
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448ER-TYPE CA2+-ATPASE 1, ATECA1, ECA1, ER-TYPE CA2+-ATPASE 1.
- Ward et al (1992).Dissociation and Reassembly of the Vacuolar H-ATPase Complex from Oat Roots. Plant Physiol. 99(1):161-9. doi: 10.1104/pp.99.1.161.
- Antibody reacts with V-ATPase subunits VHAa (100 kDa), A, B, 44 , 42, 36 , 16, 13 kDa, and with Calnexin
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