Anti-RPN5a | 26S proteasome regulatory subunit, putative (RPN5)

(Cat#: AS19 4265)
AS19_4265
AS19_4265_1
Description
  • Immunogen: Recombinant RPN5a of Arabidopsis thaliana UniProt: F4KFD7-1, TAIR: At5g09900, overexpressed in E.coli, purified from a gel piece
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Serum
  • Format: Lyophilized
  • Quantity: 50 µl
  • Reconstitution: For reconstitution add 50 µl, of sterile water
  • Storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1 : 5000 (WB)
  • Expected | apparent MW: 52.7 | 50 kDa
  • Confirmed reactivity: Arabidopsis thaliana
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • RPN5a | 26S proteasome regulatory subunit, putative (RPN5) is one of two isoforms for the 26S proteasome regulatory protein (RN) subunit RPN5. For many functions it acts redundantly with the paralogous gene RPN5b but also appears to exert independent effects.
  • Xie et al. (2024). Proteasome resides in and dismantles plant heat stress granules constitutively. Mol Cell. 2024 Sep 5;84(17):3320-3335.e7. doi: 10.1016/j.molcel.2024.07.033.Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.
  • This antibody is also recognizing RPN5b.Recommended protein load is 20 µg/well, primary antibody dilution: 1: 1000 ON/4°C and optimisation based on obtained result regarding signal/noise ratio.

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