The extraction method is based on the use of the specially designed Ron’s spin columns and an advanced buffer system. Inhibitors and other contaminants are effectively removed during the extraction process.

With this Plasmid Mini Kit, low copy number plasmids and high molecular size plasmids, including vectors such as cosmids, are easily isolated for subsequent analytical applications. This kit can also be used with high copy number plasmids in quantities (up to 20 µg) suitable for all downstream applications including sequencing and transfection.

Features

Fast, economical plasmid DNA purification
Can be used with low copy and high copy number plasmids
Specially designed spin columns with high binding capacity
Optimized buffer system for DNA recovery and removal of contaminants

Applications

PCR
Restriction enzyme digestion
Labeling
Ligation
Transformation
Transfection
Sequencing
In vitro transcription

Sizes

806942 (50 preps)

Mini spin columns x 50
2ml collection tubes x 50
Resuspension buffer PR-1, 11ml
Lysis buffer PL-2, 11ml
Neutralization buffer PN-3, 25ml
Wash buffer concentrate WB-2, 10ml
Elution buffer EL-3, 6ml
RNase A, 0.5ml

806942L (250 preps)

Mini spin columns x 250
2ml collection tubes x 250
Resuspension buffer PR-1, 55ml
Lysis buffer PL-2, 55ml
Neutralization buffer PN-3, 125ml
Wash buffer concentrate WB-2, 50ml
Elution buffer EL-3, 30ml
RNase A, 2.5ml

Literature

Manual