Ron’s Plasmid Mini Kit provides fast, simple, economical plasmid DNA purification from E. coli and other bacteria.
The extraction method is based on the use of the specially designed Ron’s spin columns and an advanced buffer system. Inhibitors and other contaminants are effectively removed during the extraction process.
With this Plasmid Mini Kit, low copy number plasmids and high molecular size plasmids, including vectors such as cosmids, are easily isolated for subsequent analytical applications. This kit can also be used with high copy number plasmids in quantities (up to 20 µg) suitable for all downstream applications including sequencing and transfection.
Features
- Fast, economical plasmid DNA purification
- Can be used with low copy and high copy number plasmids
- Specially designed spin columns with high binding capacity
- Optimized buffer system for DNA recovery and removal of contaminants
Applications
- PCR
- Restriction enzyme digestion
- Labeling
- Ligation
- Transformation
- Transfection
- Sequencing
- In vitro transcription
Sizes
806942 (50 preps)
- Mini spin columns x 50
- 2ml collection tubes x 50
- Resuspension buffer PR-1, 11ml
- Lysis buffer PL-2, 11ml
- Neutralization buffer PN-3, 25ml
- Wash buffer concentrate WB-2, 10ml
- Elution buffer EL-3, 6ml
- RNase A, 0.5ml
806942L (250 preps)
- Mini spin columns x 250
- 2ml collection tubes x 250
- Resuspension buffer PR-1, 55ml
- Lysis buffer PL-2, 55ml
- Neutralization buffer PN-3, 125ml
- Wash buffer concentrate WB-2, 50ml
- Elution buffer EL-3, 30ml
- RNase A, 2.5ml
Literature