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{{/_source.additionalInfo}}Anti-2S3P | 2S seed storage protein 3
(Cat#: AS20 4405)
Description
- Immunogen: Conjugated peptide, derived from N-propeptide of Arabidopsis thaliana 2S3P, UniProt: P15459, TAIR: At4g27160
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
- Format: Liquid at 2 mg/ml.
- Quantity: 200 µg
- Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: ELISA (ELISA), Immunolocalization (IL) using electron microscopy, Western blot (WB)
- Recommended dilutions: Assay dependent (ELISA), 1: 500 (IL), 1: 2500 (WB)
- Expected | apparent MW: 18.7 | 17-20 kDa (2S albumin precursors)
- Confirmed reactivity: Arabidopsis thaliana
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- 2S seed storage protein 3, one of major seed storage proteins is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed by an asparaginyl endopeptidase to produce two mature polypeptides referred to as large and small subunits which are linked by disulfide bonds. Subcellular localisation: vacuole.Alternative names: 2S albumin storage protein, NWMU2-2S albumin 3
- Shirakawa et al. (2014). CONTINUOUS VASCULAR RING (COV1) is a trans-Golgi network-localized membrane protein required for Golgi morphology and vacuolar protein sorting. Plant Cell Physiol. 2014 Apr;55(4):764-72. doi: 10.1093/pcp/pct195. (Western blot, Arabidopsis thaliana) Li et al. (2006). MAIGO2 is involved in exit of seed storage proteins from the endoplasmic reticulum in Arabidopsis thaliana. Plant Cell. 2006 Dec;18(12):3535-47. doi: 10.1105/tpc.106.046151.( Immunolocalisation by electron microscopy, Western blot, Arabidopsis thaliana)
- Anti-2S3P antibodies are recognizing 2S albumin precursors (p2S) but not the mature forms (2S)