Anti-Ble tag | Bleomycin resistance protein

(Cat#: AS22 4832)
AS22_4832
AS22_4832
Description
  • Immunogen: KLH-conjugated peptide derived from Bleomycin resistance protein from Streptoalloteichus hindustanus, UniProt: P17493
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Antigen affinity purified serum, in PBS pH 7.4
  • Format: Lyophilized
  • Quantity: 50 µg
  • Reconstitution: For reconstitution, add 50 µl of sterile or deionized water.
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1 : 5000 - 1: 25 000 (WB)
  • Expected | apparent MW: 13.796 kDa
  • Confirmed reactivity: Ble tag
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • The Ble tag (bleomycin resistance protein) is an important tool in molecular biology, which enables selection of cells that have successfully integrated a gene of interest. It can be used on algal, yeast, plant, bacterial and mammalian cells. By acquiring resistance to the antibiotic bleomycin, the Ble tag allows easy identification and propagation of genetically modified cells. Bleomycin is a potent antibiotic, which provides strong selection pressure that ensures only cells with the resistance gene survive.
  • To be added when available, antibody available in July 2024.

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