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{{/_source.additionalInfo}}Anti-BirA (mutated/TurboID) - HRP conjugated (40 µg)
(Cat#: AS20 4440-HRP)
Description
- Immunogen: Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP.
- Format: Liquid
- Quantity: 40 µg
- Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
- Recommended dilutions: 1 : 5000 (WB)
- Expected | apparent MW: Depends upon fusion partner
- Confirmed reactivity: BirA (mutated/TurboID)
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa.
