Bacillus subtilis Food Grade Expression Vector, 10 µg

pTTB1 (PBS041)
Sales Price$1,269.00
Please note this product requires a license for commercial companies. Please contact us for details.

The Bacillus subtilis system was developed as a host for gram-positive bacteria in a variety of applications, including agricultural, medical and food biotechnology and the production of recombinant proteins. 

The Bacillus subtilis system is advantageous in that it is non-pathogenic, does not have significant bias in codon usage, and is capable of secreting functional extracellular proteins directly into the culture medium. A large body of information concerning transcription, translation, protein folding and secretion mechanisms, genetic manipulation, and large-scale fermentation is available.

The Bacillus Food Grade Expression System was created to make the advantages of the Bacillus expression system also accessible to areas in which antibiotic resistance gene markers are prohibited, such as the food and feed industry. It enables stable vector-based large scale heterologous protein production by an alternative selection, without antibiotics.

The Bacillus Food Grade Selection System is based on the interplay of an endogenous Bacillustoxin EndoA (expressed from vector) and its antitoxin EndoB (expressed from genome). 

Two vectors are available for this system, differing in their origin of replication and copy number concerning B. subtilis:

  • pTTB1 (PBS041) - low copy number vector; replicates via theta replication modus 
  • pTTB1 (PBS042) - high copy number vector; replicates by rolling circle mechanism

For easy handling, the vectors are designed as B. subtilis / E. coli shuttle vectors. The parts of the vector used for cloning with E. coli can be eliminated afterwards by restriction enzyme cleavage and religation of the vector. This technique connects the advantage of easy cloning (with E. coli) with the food grade property of B. subtilis

Please note these products require a license for commercial companies. Please contact us for licensing information.


  • Stable high- or low-level expression without addition of any antibiotics
  • All DNA contained in the final expression system is derived from B. subtilis
  • Cloning can be done with E. coli
  • No endotoxins are produced
  • No inclusion bodies are formed
  • Host for gram-positive bacteria in food production
  • PBS041 - pTTB1 vector, lyophilized DNA, 10 µg
  • PBS042 - pTTB2 vector, lyophilized DNA, 10 µg

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