MethylDetect and DNA Methylation Citations
- Daugaard I, Kjeldsen TE, Hager H, et al. The influence of DNA degradation in formalin-fixed, paraffin-embedded (FFPE) tissue on locus-specific methylation assessment by MS-HRM. Experimental and Molecular Pathology. 2015;99(3): 632-640. doi: doi.org/10.1016/j.yexmp.2015.11.007
- Heitzer E, Perakis S, Geigl JB, Speicher MR The potential of liquid biopsies for the early detection of cancer. NPJ Precis Oncol. 2017;1(1):36. Published 2017 Oct 17. doi:10.1038/s41698-017-0039-5
- Kristensen, L.S., Wojdacz, T.K., Thestrup, B.B. et al. Quality assessment of DNA derived from up to 30 years old formalin fixed paraffin embedded (FFPE) tissue for PCR-based methylation analysis using SMART-MSP and MS-HRM. BMC Cancer. 2009; 9, 453. doi:10.1186/1471-2407-9-453
- Lønning PE, Nikolaienko O, Pan K, et al. Constitutional BRCA1 Methylation and Risk of Incident Triple-Negative Breast Cancer and High-grade Serous Ovarian Cancer. JAMA Oncol. 2022;8(11):1579–1587. doi: doi.org/10.1001/jamaoncol.2022.3846
- Łuczkowska K, Sokolowska KE, Taryma-Lesniak O, et al. Bortezomib induces methylation changes in neuroblastoma cells that appear to play a significant role in resistance development to this compound. Sci Rep. 2021;11(1):9846. Published 2021 May 10. doi:10.1038/s41598-021-89128-0
- Machaj F, Sokolowska KE, Borowski K, et al. Analytical sensitivity of a method is critical in detection of low-level BRCA1 constitutional epimutation. Sci Rep. 2023; (13). doi: 10.1038/s41598-023-43276-7
- Mathiasen S.S, Bińkowski J, Kjeldsen T, et al. Methylation levels assessment with Methylation-Sensitive High-Resolution Melting (MS-HRM). PloS one. 2022;17(9): e0273058. doi: 10.1371/journal.pone.0273058
- Tang W, David FB, Wilson MM, Barwick BG, Leyland-Jones BR, Bouzyk MM. DNA extraction from formalin-fixed, paraffin-embedded tissue. Cold Spring Harb Protoc. 2009;2009(2):pdb.prot5138. doi:10.1101/pdb.prot5138
- The BLUEPRINT consortium. Quantitative comparison of DNA methylation assays for biomarker development and clinical applications. Nat Biotechnol. 2016;34:726–737. Published 2021 May 10. doi:10.1038/nbt.3605
- Warnecke PM, Stirzaker C, Melki JR, Millar DS, Paul CL, Clark SJ. Detection and measurement of PCR bias in quantitative methylation analysis of bisulphite-treated DNA. Nucleic Acids Res.1997;25(21):4422-4426. doi:10.1093/nar/25.21.4422
- Wojdacz TK, Dobrovic A. Methylation-sensitive high resolution melting (MS-HRM): a new approach for sensitive and high-throughput assessment of methylation. Nucleic Acids Res. 2007;35(6):e41. doi:10.1093/nar/gkm013
- Wojdacz TK, Dobrovic A. Melting curve assays for DNA methylation analysis. Methods Mol Biol. 2009;507:229-240. doi:10.1007/978-1-59745-522-0_17