Home New Customer? Create Account
Member Login:

DNA Polymerases/SNPase, dNTPs & Kits

101004/101024 - DFS HotTaq DNA Polymerase

PRICE

Starting at: $86.00

Please Choose:

Size



Add to Cart:

DFS HotTaq DNA Polymerase is an optimized mixture of DFS-Taq DNA Polymerase and Anti-Taq monoclonal antibodies. Antibodies block polymerase activity during set-up of the PCR reactions at ambient temperature. The inhibition of DFS HotTaq DNA polymerase is completely reversed when the temperature is above 70°C.

DFS HotTaq Polymerase catalyzes the polymerization of nucleotides into duplex DNA in 5’ – 3’ direction in the presence of magnesium ions and possesses a 5’ – 3’ exonuclease activity.

This DNA-free polymerase is highly purified and is free of nonspecific endo- or exonucleases.

 

Features

  • Free from bacterial DNA
  • Amplifies fragments up to 5kb
  • Universal buffer system for different applications
  • High sensitivity
  • Efficient amplification of single copy genes
  • High product yield

 

Applications

  • Amplification of DNA fragments up to 5kb
  • Amplification of single-copy genes
  • Labeling of PCR products with modified nucleotides 
  • Cycle sequencing
  • Generation of PCR products for TA-cloning
  • Detection of bacterial pathogens

 

Sizes

101004 (500 U)

  • DFS HotTaq DNA Polymerase, 5 U/µl, 500 U
  • Incomplete NH4 Reaction Buffer (10x), 1.8ml
  • Complete NH4 Reaction Buffer (10x), 1.8ml
  • Complete KCl Reaction Buffer (10x), 1.8ml
  • MgCl2 100 mM, 1ml

101024 (2500 U)

  • DFS HotTaq DNA Polymerase, 5 U/µl, 2500 U
  • Incomplete NH4 Reaction Buffer (10x), 9ml
  • Complete NH4 Reaction Buffer (10x), 9ml
  • Complete KCl Reaction Buffer (10x), 9ml
  • MgCl2 100 mM, 5ml

 

Literature