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DNA Polymerases/SNPase, dNTPs & Kits

118710/118750 - Single Cell Whole Genome Amplification Kit

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Starting at: $203.00

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The Single Cell Whole Genome Amplification (WGA) Kit contains everything that is necessary for amplification of human genomic DNA from single cells or low copy templates. This kit is an improved version of DOP (degenerated oligo PCR) with optimized primer sequences and our unique SD Polymerase

With the WGA kit, amplification is based on a multiple displacement reaction in combination with specially designed degenerated primers optimized for human genomic DNA. The included SD Polymerase is able replace former strands during the synthesis of the new strand. Due to its the temperature stability up to 92°C, the two common reaction steps within a whole genome amplification assay do not require additional handling time. This minimizes the risk of contamination and handling errors.

 

Features

  • Amplification can be completed in less than 2 hours
  • Different genomic amplification coverage than other enzymes
  • Long fragments up to 3 Kb
  • Only uses one enzyme for the whole genome amplification

 

Applications

  • Single cell DNA analysis
  • Next Generation Sequencing (NGS)
  • PID
  • SNP Genotyping
  • Allele Dropout
  • qPCR- and PCR-based mutation detection
  • STR and RFLP analysis
  • Array technologies (comparative genomic hybridization)

 

Sizes

118710 (10 reactions)

  • SD Polymerase, 10 U/µl, 10µl
  • 2.5x Master Mix without enzyme, 100µl
  • PCR grade water, 1800µl
  • Single Cell Lysis Buffer, 30µl

118750 (50 reactions)

  • SD Polymerase, 10 U/µl, 50µl
  • 2.5x Master Mix without enzyme, 500µl
  • PCR grade water, 1800µl
  • Single Cell Lysis Buffer, 150µl

 

Literature