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{{/_source.additionalInfo}}e-Myco™ VALiD-Q Real-time PCR Kit
Cat#: 25246
Description
e-Myco VALiD-Q Real-time PCR Kit (ver2.0) is specifically designed to detect mycoplasma by targeting highly conserved regions of Mycoplasma DNA.i
The e-Myco™ VALiD-Q Real-time PCR Kit (ver. 2.0) is suitable for rapid and accurate testing of mycoplasma negative results, providing confirmation in under 1 hour (52 minutes).
The e-Myco™ VALiD-Q Real-time PCR Kit (ver. 2.0) is an in vitro nucleic acid amplification test optimized for the detection of Mycoplasma in K562 cell culture, according to the Nucleic Acid Amplification Tested (NAT) guidelines for Mycoplasma described in chapter 2.6.7 of the European Pharmacopoeia, with respect to specificity, sensitivity/detection limit, and robustness.
The e-Myco™ VALiD-Q Real-time PCR Kit (ver. 2.0) is suitable for rapid and accurate testing of mycoplasma negative results, providing confirmation in under 1 hour (52 minutes).
The e-Myco™ VALiD-Q Real-time PCR Kit (ver. 2.0) is an in vitro nucleic acid amplification test optimized for the detection of Mycoplasma in K562 cell culture, according to the Nucleic Acid Amplification Tested (NAT) guidelines for Mycoplasma described in chapter 2.6.7 of the European Pharmacopoeia, with respect to specificity, sensitivity/detection limit, and robustness.
- Amplification : The extracted DNA is amplified using a 5’nuclease fluorescent probe and specific primer pairs.
- Target Regions : The assay targets two specific regions: Mycoplasma (FAM) and Internal Positive Control (IPC) (HEX).
- Purpose of IPC : The IPC is included in the kit to confirm the success of the Real-time PCR reaction and is co-amplified with the target band from test samples
Analysis Workflow:
Sensitivity:
- Amplifies Mycoplasma with high sensitivity and specificity
- Rapid contamination detection using real-time PCR
- Able to detect over 70 species of Mycoplasma
- Suitable for monitoring the manufacturing process of pharmaceuticals in the field of cell therapy and monitoring contamination in cultured cell lines
- Validation of a PCR method for the detection of Mycoplasmas according to European Pharmacopoeia section 2.6.7
- Mycoplasma detection in cell cultures
- Mycoplasma validation for the production of biopharmaceuticals
- Prevention of carry-over contamination using Uracil-DNA Glycosylase (UDG)
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Application of IPC system to verify the validity of PCR
- 2X qPCR Master Mix Solution, 280 μl x 2 vials
- Mycoplasma Detection Solution, 140 μl x 2 vials
- Positive control DNA, 25µl x 3 vials
- DNase/RNase free water, 1ml
G-spin Total DNA Extraction Kit
e-Myco VALID Mycoplasma PCR Detection KitBoca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
