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{{/_source.additionalInfo}}NICE® Lactococcus lactis Expression Vector
pNZ8148 (ELV00200-01)
Sales Price$1,205.00
Please note this product requires a license for commercial companies. Please contact us for details.
Description
The Nisin controlled gene expression system (NICE®), developed at NIZO Food Research, NL, is a fully food grade system used for controlled gene expression in Lactococcus lactis. The NICE® system is advantageous in that it does not contain endotoxins, inclusion bodies, spores or extracellular proteases, and the tightly controlled gene expression allows the production of toxic proteins to take place. With this system, fermentation, scale-up and downstream processing are simple.
Vectors
- pNZ8148 (ELV00200-01) - broad-host-range vector; CmR; nisin A promoter (PnisA) followed by an NcoI site for translational fusions at the ATG. Contains a terminator after the MCS. Sequence adaptation for cloning into NcoI site can result in a change in the second amino acid of a protein.
- pNZ8149 (ELV00300-01) - broad-host-range vector; lacF for food grade selection for growth on lactose; nisA promoter followed by an NcoI site for translational fusions at the ATG. Contains a terminator after the MCS. Sequence adaptation for cloning into NcoI can result in a change in the second amino acid of a protein.
- pNZ8150 (ELV00250-01) - broad-host-range vector; CmR; nisA promoter followed by ScaI site for translational fusions precisely at the ATG. Blunt end fragments are generated by PCR and cannot be cut out again after ligation to the ScaI site. Contains a terminator after the MCS.
- pNZ8151 (ELV00310-01) - broad-host-range vector; lacF gene as food grade selection marker; nisA promoter followed by an ScaI site for translational fusions at the ATG start codon. Contains a terminator after the MCS.
- pNZ8152 (ELV00400-01) - broad-host-range vector; alr gene as food grade selection marker, nisA promoter followed by an ScaI site for translational fusion at the ATG. Contains a terminator after the MCS.
- pNZ8008 (ELV00100-01) - reference plasmid for testing the nisin induction in Lactococcus and other lactic acid bacteria genera. A gusA gene without promoter was fused to the nisin A promoter.
- pNZ9530 (ELV00500-01) - low copy plasmid with pAMβ1 origin of replication, which carries the nisR and nisK genes. For cloning in Lactococcus strains and in strains of other lactic acid bacteria genera that do not have the regulatory genes integrated into the chromosome. In this case, for nisin induced expression a dual plasmid system is used: e.g., pNZ9530 (nisRnisK) + pNZ8150 (+insert).
See also: NICE® Lactococcus lactis Secretion Vectors
Please note a license is required for commercial companies. Please contact us for licensing details.
- Endotoxin-free, food grade expression system
- Ideal for expression of membrane proteins and secretion of proteins into the medium
- Fewer endogenous and no exogenous proteases
- No spores or inclusion bodies
- Tightly controlled gene expression allows production of toxic proteins
- Simple fermentation, scale-up and downstream processing
- Production of homologous and heterologous proteins for food, feed, pharmacological and biocatalysis applications
- Production of prokaryotic and eukaryotic membrane proteins
- Production of exopolysaccharides
- Production of ingredients through metabolic engineering: e.g. alanine, folate, diacetyl
- Preparation of L. lactis as a biocatalyst by expression of a suitable enzyme as, e.g., dehydrogenases and in-situ cofactor regeneration
- High-throughput screening for enzyme evolution or enzyme comparison
| Cat# | Vector | Note |
| ELV00100-01 | pNZ8008 | gusA gene |
| ELV00200-01 | pNZ8148 | NcoI site |
| ELV00250-01 | pNZ8150 | ScaI site |
| ELV00300-01 | pNZ8149 | Food grade, NcoI site |
| ELV00310-01 | pNZ8151 | Food grade (lacF), ScaI site |
| ELV00400-01 | pNZ8152 | Food grade (alr), ScaI site |
All vectors are supplied as lyophilized DNA (10µg).
NICE® Lactococcus lactis Host Strain
NICE® Lactococcus lactis Secretion Vector
NICE® pNZ9530 Lactococcus lactis nisRnisK vector, in Strain NZ9000Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
