Transfection Reagents
1. What is the stability ScreenFect® transfection reagents?
If kept refrigerated at 4°C, ScreenFect® transfection reagents' efficiency is guaranteed for at least 12 months. For optimal long term activity, remove from 4°C only for short periods when required for dilution in the provided buffer. Do not freeze the reagents. There is no significant loss of activity with storage at room temperature for two weeks, allowing room temperature shipment.
2. How do I store ScreenFect® transfection reagents?
ScreenFect® transfection reagents should be stored at 4°C. Do not leave for extended periods at room temperature and do not freeze.
3. How do you calculate transfection efficiency?
Different methods can be used for the quantification of both efficiency and toxicity, such as fluorescent microscopy, flow cytometry, luminescence readouts.
4. Which Screenfect product should be used for your transfection?
ScreenFect reagents successfully deliver pDNA, siRNA, microRNA, and CrispR. ScreenFect A should be used for CrispR and Cas9 RNP. For pDNA transfections, ScreenFect A can be used for ScreenFect A-Plus. For more standard celllines like Hela, Hek as no optimization is necessary. ScreenFect siRNA should be used for siRNA transfections.