Luria Broth (Miller's LB Broth)

Cat#: GCM04.0500
gcm04.0500
Sales Price$95.00
Size
500g
Description

Luria Broth (Miller’s Modification) is a dehydrated powder for the preparation of nutritionally rich liquid medium for the growth and maintenance of recombinant strains of E. coli in molecular biology studies.

This rich growth medium contains all the nutritional requirements for E. coli. Tryptone and Yeast Extract are the sources for carbon, nitrogen, vitamins, minerals, and amino acids essential for growth, while sodium chloride supplies essential electrolytes for transport and osmotic balance. For faster growth, the medium can be supplemented with glucose (0.1%) or glycerol (0.4%). Many supplements, including antibiotics, are heat-sensitive and cannot be autoclaved. These should be filter-sterilized and added to the medium after it has cooled down.

Formulation (g/L)

  • Tryptone: 10.00
  • Yeast Extract: 5.00
  • NaCl: 0.50
  • Final pH (25°C): 7.0 ± 0.2

Preparation

Add 25g of the dehydrated medium to one liter of distilled water. Mix well and dissolve by heating with regular agitation. Boil for 1 minute in order to dissolve completely. Dispense in appropriate containers and sterilize by autoclaving at 121°C for 15 to 20 minutes. Store at 2-8°C.

 

  • Contains all the nutritional requirements for E. coli
  • Prepares liquid medium
  • Luria Broth (Miller’s LB Broth), 500g

Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.

Recently Viewed Products