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Culture Media

GCM12.0500 - SOC Medium, 500g

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$89.00

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SOC Medium is a dehydrated powder for the preparation of nutritionally rich liquid medium for the preparation and transformation of E. coli. Transformation requires perforation of bacteria to allow DNA to enter the cell. To survive this process, competent cells need isotonic rich media. SOC Medium is well suited for this role. 

SOC contains all the nutritional requirements for E. coli. Tryptone and Yeast Extract are the sources for carbon, nitrogen, vitamins, minerals, and amino acids essential for growth. Sodium chloride and Potassium chloride supply essential electrolytes for transport and osmotic balance, while Magnesium sulfate serves as a source for magnesium ions. Glucose is used as an additional carbon and energy source for repair of the perforation and for replication.

Formulation (g/L)

  • Tryptone: 20.00
  • Yeast Extract: 5.00
  • NaCl: 0.50
  • MgSO4: 0.96
  • Glucose: 3.60
  • KCl: 0.186
  • Final pH (25°C): 7.0 ± 0.2

Preparation
Add 30.2g of the dehydrated medium to one liter of distilled water. Mix well and dissolve by heating with regular agitation. Boil for 1 minute in order to dissolve completely. Dispense in appropriate containers and sterilize by autoclaving at 121°C for 15 to 20 minutes. Store at 2-8°C.

 

Features

  • Optimized for the preparation and transformation of competent cells 
  • Isotonic rich medium
  • Contains all the nutritional requirements for E. coli
  • Glucose is used as an additional carbon and energy source

 

Contents

  • SOC Medium, 500g