Yeast Nitrogen Base (without added amino acids and WITH ammonium sulfate)

Yeast Nitrogen Base (without added amino acids and with ammonium sulfate) is a dehydrated powder for the preparation of YNB liquid medium. This medium is used for the classification of yeast based on their carbon and/or nitrogen requirements. It contains all the essential ingredients required for the cultivation of yeast, except for the amino acids, a nitrogen source and a carbohydrate source.

Formulation (g/L)

• YNB: 1.70
• Ammonium sulfate: 5.00
• Final pH (25°C): 5.4 ± 0.2

Trace Elements (mg/L)

• Inositol: 2.0
• Boric Acid: 0.5
• Manganese Sulfate: 0.4
• Potassium Iodide: 0.1
• Niacin: 0.4
• Pyridoxine HCL: 0.4
• Z_nSO₄: 0.4
• Sodium Molybdate: 0.2
• Thiamide HCL: 0.4
• Calcium Pantothenate: 0.4
• Ferric Chloride: 0.2
• Biotin: 0.002
• Copper Sulfate: 0.04
• p-Aminobenzoic acid: 0.2
• Riboflavin: 0.2
• Folic Acid: 0.002
• Monopotassium Phosphate: 1.0g
• Calcium Chloride: 0.1g
• Sodium Chloride: 0.1g