Description
Yeast Nitrogen Base (without added amino acids and without ammonium sulfate) is a dehydrated powder for the preparation of YNB liquid medium. This medium is used for the classification of yeast based on their carbon and/or nitrogen requirements. It contains all the essential ingredients required for the cultivation of yeast, except for the amino acids, a nitrogen source and a carbohydrate source.
Formulation (g/L):
- YNB: 1.70
- Final pH (25°C): 4.5 ± 0.2
Trace Elements (mg/L):
- Inositol: 2.0
- Boric Acid: 0.5
- Manganese Sulfate: 0.4
- Potassium Iodide: 0.1
- Niacin: 0.4
- Pyridoxine HCL: 0.4
- ZnSO4: 0.4
- Sodium Molybdate: 0.2
- Thiamide HCL: 0.4
- Calcium Pantothenate: 0.4
- Ferric Chloride: 0.2
- Biotin: 0.002
- Copper Sulfate: 0.04
- p-Aminobenzoic acid: 0.2
- Riboflavin: 0.2
- Folic Acid: 0.002
- Monopotassium Phosphate: 1.0g
- Calcium Chloride: 0.1g
- Sodium Chloride: 0.1g
- Magnesium sulfate: 0.5g
- Prepares YNB liquid medium used for classifying yeast
- Yeast Nitrogen Base (without added amino acids and without ammonium sulfate), 500g