Assay Defender®

50ml (Cat# 180 050)
180-assay-defender
Sales Price$86.00
Size 
Description

Total HAMA and Interference Blocker.

Assay Defender® is a ready-to-use sample dilution buffer that reduces multiple types of interference.  It is ideal for immunoassays with biological samples from humans and animals in which interference caused by HAMA (human anti-mouse antibodies), AAA (anti-animal antibodies) or other high-affinity antibodies can occur.

Assay Defender® reduces interference caused by:

  • HAMA 
  • Rheumatoid factors
  • Nonspecific binding
  • Cross-reactivities 
  • Matrix effects

While ordinary HAMA blockers only work against interference caused by HAMA and other interfering antibodies, Assay Defender® eliminates interference from all sources.  This elimination optimizes the assay and helps prevent false positive or false negative results.

With its multiple functions as a diluent and optimizer, Assay Defender® prevents time and cost-intensive optimization steps such as HAMA blocker selection, extended titration experiments or addressing each interference source individually. 

When using Assay Defender®, calibrators must be diluted in this buffer. Dilution in Assay Defender® can be 1:2 or higher. A useful dilution factor for most applications is 1:10 (1 part specimen + 9 parts Assay Defender®). 

 

Assay Defender® vs. LowCross-Buffer®

Both Assay Defender® and LowCross-Buffer® contain the special "LowCross® effect" that allows them to prevent interference based on low and medium affinity binding. Interference from high affinity antibodies, however, can only be eliminated by Assay Defender®. Additionally, there are applications where LowCross Buffer may be the more appropriate choice.  Learn more about the differences between Assay Defender® and LowCross-Buffer®.

 

  • Ideal for human or animal specimens containing HAMA or other AAA 
  • Reduces multiple types of interference, not only HAMA as with typical HAMA blockers
  • Eliminates interference from high affinity antibodies 
  • Helps prevent false positives and false negatives
  • Easy to use, avoids the need for additional optimization steps
  • ELISA
  • Immuno-PCR
  • Lateral Flow Assay
  • Multiplex Assay (such as bead-based and protein-based)
  • 180 050 - Assay Defender®, 50ml
  • 180 125 - Assay Defender®, 125ml
  • 180 500 - Assay Defender®, 500ml

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