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Immunoassay Optimization Buffers

100 000 - LowCross-Buffer®


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Antibody and sample diluent for minimizing nonspecific binding, cross-reactivities and matrix effects in immunoassays.

LowCross-Buffer® is an innovative assay buffer for ELISA, EIA, RIA, protein arrays, Western blotting, immunohistochemistry and immuno-PCR. It is ready-to-use and can replace your standard sample or antibody dilution buffer.

The formulation of LowCross-Buffer® helps to reduce immunoassay interference and can therefore greatly improve the quality of detection. Nonspecific binding of the antibodies, negative effects of interfering substances, and low or medium affinity cross reactivities of the antibodies can be significantly decreased. Matrix effects, such as those from blood sera or plasma specimens, are minimized. Reduction of these negative effects improves the quality of the assay and the reliability of the results.



  • Improves quality of results and reliability of assay
  • Reduces interference caused by HAMAs (Human Anti-Mouse Antibodies) and rheumatoid factor
  • Can be used with both monoclonal and polyclonal antibodies (concentrations may need adjusting)
  • Can be used in combination with fluorescent dyes
  • Does not replace blocking buffer; we recommend The Blocking Solution
  • Free of phosphate


  • Western blotting
  • Protein Arrays
  • Immuno-PCR
  • Immunohistochemistry
  • Enzyme immunoassay
  • Radioimmunoassay


  • 100 050 – LowCross Buffer, 50ml
  • 100 125 – LowCross Buffer, 125ml
  • 100 500 – LowCross Buffer, 500ml





100000, 100050, 100-050, 100125, 100-125, 100500, 100-500, low-cross

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