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V33002 - Standard Cloning Vector pUC18
PRICE
Starting at: $129.00
Please Choose:
Add to Cart:
pUC18 is a small, high copy cloning vector for replication in
E. coli
. It has been constructed using the ampicillin resistance gene and the pMB1 origin of replication from
pBR322
. The pMB1 of pUC18 differs from the pBR322 origin by a single point mutation and the lack of the
rop
gene, leading to a high copy number. pUC18 has a multiple cloning site within the lacZ alpha-fragment. Inserts cloned into this site disrupt beta -galactosidase activity and give rise to white colonies on X-Gal/IPTG plates. Foreign DNA inserted in-frame with the lacZ gene will be expressed as a fusion protein (containing a portion of the beta-galactosidase) under control of the lac promoter. The promoter is inducible with IPTG and followed by an initiation codon as well as a ribosome binding site. pUC18 differs from
pUC19
in the multiple cloning site orientation.
Features
Resistant to ampicillin
Multiple cloning site located within lacZ
Origin = pMB1
Contents
pUC18 Vector DNA, 25 µg
Literature
Product Information Sheet