Additive for increased efficiency in hybridoma production.
BriClone is an additive for the cloning medium used in the post-fusion stages of hybridoma cell cloning. It has been specifically developed by the NICB's dedicated team to overcome common problems in the post-fusion period, such as batch-to-batch variation of feeder cells and competition between feeder cells and freshly-fused hybridomas for nutrients in the culture medium.
BriClone is intended for use when cloning hybridoma cell lines under conditions of limiting dilution. This conditioned medium was collected from a proprietary human cell line that produces IL-6. Each batch is tested for its ability to support the outgrowth of hybridomas from freshly fused cells and for its ability to support the growth of hybridomas under conditions of limiting dilution.
To use BriClone, add to your hybridoma culture media as a 5% (v/v) supplement. Use of this product eliminates the need for feeder cells in these types of cultures.