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Constitutive Gene Expression System for Lactococcus lactis and Other Lactic Acid Bacteria

ELS03000 - Lactococcus lactis expression strain NZ3000, food grade, 1ml

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The Constitutive Gene Expression System for Lactococcus lactis and other lactic acid bacteria, developed at NIZO Food Research, NL, is a food-grade protein expression system. This system is advantageous in that it does not contain endotoxins, inclusion bodies, spores or extracellular proteases, and can be used for bacteria other than Lactococcus lactis. With this system, fermentation, scale-up and downstream processing are simple.
 
The Lactococcus lactis expression strain NZ3000 is a standard strain for food-grade selection based upon the ability to grow on lactose. The lactose operon that is generally present on plasmids has been integrated into the chromosome and the lacF gene was deleted. Deletion of the lacF gene makes this strain unable to grow on lactose unless lacF is provided on a plasmid.
 
Please note this product requires a license for non-academic institutions. Please contact us for licensing information.
 

Features

  • Endotoxin-free, food-grade expression system
  • Fewer endogenous and no exogenous proteases
  • No spores or inclusion bodies 
  • Simple fermentation, scale-up and downstream processing
  • Suitable for bacteria other than Lactococcus lactis
 

Applications

  • Overexpression of homologous and heterologous genes for functional studies and to obtain large quantities of specific gene products
  • Metabolic engineering
  • Protein secretion and anchoring in cell envelope
  • Large scale applications
 

Contents

  • Lactococcus lactis expression strain NZ3000, food-grade, 1ml
 

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