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Bacillus megaterium T7 RNA Polymerase High Yield Gene Expression System

BMEGT711 - Bacillus megaterium pPT7-SPlipA secretion vector, lyophilized, 10 µg

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The vector pPT7-SPlipA is a secretion vector for the T7 RNA Polymerase high yield gene expression system for Bacillus megaterium. pPT7-SPlipA enables extracellular protein production. It provides the Lipase A signal sequence that leads to secretion of the protein of interest into the medium.

About the T7 RNA polymerase expression system
 
The Bacillus megaterium high yield T7 gene expression system combines the features of the B. megaterium system in E. coli with the T7 RNA polymerase expression system regulation by the xylose operon.
 
This dual plasmid system is based on two parallel-replicating plasmids: pT7-RNAP and pPT7pT7-RNAP contains the genes for ampicillin and chloramphenicol resistance for easy selection in E. coli (AmpR) and B. megaterium (CmR), in addition to the t7 rnap gene under control of the strong xylA promoter. pPT7 is responsible for the T7 RNAP-dependent expression of the target gene. Downstream of the T7 promoter, it comprises a multiple cloning site with ten unique restriction enzyme cleaving sites. Additionally, pPT7 comprises two resistances against ampicillin (in E. coli) and tetracycline (in B. megaterium).
 
B. megaterium has proven to be an excellent host for the expression of non-homologous DNA. Unlike other bacilli strains, none of the alkaline proteases are present and no endotoxins are found in the cell wall. B. megaterium can stably maintain several extra-chromosomal DNA elements in parallel. Protein yields are exceptionally high, even if inexpensive substrates are used. 
 
Please note this product requires a license for non-academic institutions. Please contact us for licensing information.
 

Features

  • Stable, high yield protein production up to 8-fold increase compared to common xylose inducible expression
  • Enables extracellular protein production
  • Tightly regulated and efficiently inducible xylA operon/T7 RNA polymerase
  • Suitable for cloning of toxic proteins in E. coli
  • Ideal for both small- and large-scale protein production
  • B. megaterium is not pathogenic, no endotoxins found in cell wall
 

Contents

  • B. megaterium pPT7-SPlipA secretion vector, lyophilized, 10 µg
 

Literature