Bacillus subtilis SURE System Vector, 10µg

The SURE® System: Subtilin Regulated gene Expression

Bacillus subtilis is an attractive host for gram-positive bacteria as it is non-pathogenic, does not have significant bias in codon usage, and is capable of secreting functional extracellular proteins directly into the culture medium. It is used in a variety of applications, including agricultural, medical and food biotechnology and the production of recombinant proteins.

Controlled gene expression in B. subtilis is a cost-effective alternative to IPTG induced gene expression. This is achieved with the used of subtilin, a small peptide antibiotic of 32 amino acids produced by B. subtilis. Subtilin production and regulation is encoded in the chromosome by a cluster of 9 genes, which are transcribed from two promoters. Subtilin regulates/activates its own biosynthesis via a two-component regulatory system.

SURE System Vectors

• pNZ8901 (PBS031) - SURE expression vector, PspaSmut, Cm^R; high promoter activity, but some leakage; suitable for the production of non-toxic proteins. 
• pNZ8911 (PBS032) - SURE expression vector, PspaS, Cm^R; no promoter activity without subtilin induction, lower expression than PspaSmut; suitable for the production of potentially toxic proteins. 
• pNZ8902 (PBS033) - SURE expression vector, PspaSmut, Em^R; high promoter activity, but some leakage; suitable for the production of non-toxic proteins. 
• pNZ8910 (PBS034) - SURE expression vector, PspaS, Em^R; some promoter leakage without subtilin induction, caused by the vector; lower expression than pNZ8911.

Several B. subtilis strains specifically developed for the SURE system are available as well. Additionally, E. coli strain MC1061 can be used with the SURE system.

Please note these products require a license for commercial companies. Please contact us for licensing information.