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Bacillus subtilis Protein Expression System

BSUB FOOD GRADE STRAINS - Bacillus subtilis food grade strains, 1ml

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Starting at: $1,300.00

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The Bacillus subtilis system was developed as a host for gram-positive bacteria in a variety of applications, including agricultural, medical and food biotechnology and the production of recombinant proteins.

The Bacillus subtilis system is advantageous in that it is non-pathogenic, does not have significant bias in codon usage, and is capable of secreting functional extracellular proteins directly into the culture medium. A large body of information concerning transcription, translation, protein folding and secretion mechanisms, genetic manipulation, and large-scale fermentation is available.

The Bacillus Food Grade Expression System was created to make the advantages of the Bacillus expression system also accessible to areas in which antibiotic resistance gene markers are prohibited, such as the food and feed industry. It enables stable vector-based large scale heterologous protein production by an alternative selection, without antibiotics.

The Bacillus Food Grade Selection System is based on the interplay of an endogenous Bacillustoxin EndoA (expressed from vector) and its antitoxin EndoB (expressed from genome). EndoA is an endoribonuclease that specifically cleaves mRNA at a five Base U↓ACAU sequence. During normal growth conditions, EndoA is inactivated by forming a heterohexameric complex with its cognate antitoxin, EndoB. Since the antitoxin is relatively unstable, it is essential for the cell to continuously produce sufficient amounts of EndoB to inactivate the more stable toxin. These characteristics are utilized for retaining the vector within the cell.

Two host strains are available for this system:

  • TEA (PBS043) - based on B. subtilis 168 Marburg; recommended for intracellular protein expression and pathway engineering.
  • WEA (PBS044) - originated from the eightfold extracellular protease-deficient strain B. subtilis WB800N; particularly constructed for secretory protein production.

Both strains are suitable hosts for both the food grade low copy expression vector pTTB1 and the high copy expression vector pTTB2.

Please note these products require a license for non-academic institutions. Please contact us for licensing information.

Features

  • Stable high- or low-level expression without addition of any antibiotics
  • All DNA contained in the final expression system is derived from B. subtilis
  • No endotoxins are produced
  • No inclusion bodies are formed

 

Applications

  • Host for gram-positive bacteria in food production

 

Strains

  • PBS043 - strain TEA, 1ml
  • PBS044 - strain WEA, 1ml

 

Literature

 

See also: B. subtilis food grade expression vectors