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E. coli Expression Systems

V32802 - Standard Cloning Vector pBR328


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pBR328 is an ampicillin, tetracycline and chloramphenicol resistant, general purpose cloning vector. It is a derivative of pBR325 produced by the deletion of the bom (basis of mobility) site. Due to this deletion, pBR238 is non-mobilizable, making it suitable for situations that require more stringent biological containment. This deletion creates additional unique cloning sites in the chloramphenicol acetyltransferase gene: Pvu II, BspM II and Bal I.


  • Resistant to ampicillin, tetracycline and chloramphenicol
  • Non-mobilizable derivative of pBR325
  • Origin = ColE1


  • pBR328 Vector DNA, 25 µg