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Mycoplasma Detection

25237 - e-Myco PLUS Mycoplasma PCR Detection Kit, 48 tests

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The e-Myco™ PLUS Mycoplasma PCR Detection Kit consists of a set of primers designed to detect the presence of mycoplasma and determine the species. This kit can detect 209 species of mycoplasma over 8 genus. The species of mycoplasma can be determined by sequencing the amplified products. 
 
Mycoplasma is a common and serious contaminant of cell cultures. It has been shown that 30-87% of cell cultures are infected with Mycoplasma. In continuous cell cultures, contaminating Mycoplasma may grow slowly, not killing the cells outright but affecting various parameters, leading to unreliable results. Testing for Mycoplasma is an important and necessary quality control measure. 
 
The primers used in this Mycoplasma detection kit anneal to conserved regions of the Mycoplasma genome, allowing detection of all important species of Mycoplasma. Mycoplasma contaminants can be detected simply as amplified fragments on a DNA gel; labeled probes are not necessary.
 
Each e-Myco PLUS Mycoplasma PCR detection tube contains all components required for PCR in a dried and stable pellet format, including i-StarTaq DNA Polymerase, dNTPs, 10x Buffer, Mycoplasma primers, 8-methoxypsoralen, and an internal control for Mycoplasma partial gene amplifications. The reaction can begin by simply adding the template DNA or samples to be tested. PCR with this primer set is based on conserved 16S rRNA and delivers detection results in only a few hours. This is far more efficient than the conventional detection methods that involve culturing samples on selective media and require at least 1 week to obtain results. 
 
To ensure accurate results, e-myco PLUS includes a triple checking system:
  1. Internal control (embedded in each tube) to reduce the chance of false negatives 
  2. Sample control (embedded in each tube) to test the integrity of the template DNA 
  3. Separate positive control (in separate tube) to validate the assay
 

Features

  • Tubes contain all components required for PCR in a dried and stable pellet format 
  • Includes triple checking system: Internal control, sample control and positive control 
  • 8-methoxypsoralen solution prevents carryover contamination by PCR products
  • Highly sensitive due to Hot-start Taq DNA Polymerase; detects 15 infected cells or 6.3pg genomic DNA
  • Species of mycoplasma can be determined by sequencing the amplified products
  • Detects 209 species of Mycoplasma
  • Gel loading dye premixed to direct electrophoresis
  • High stability and reproducibility; vacuum packaging prevents oxidation and humidity
 

Applications

  • Mycoplasma detection
 

Contents

  • e-Myco™ Mycoplasma PLUS PCR Detection Tubes* x 48
  • DNase/RNase-free Distilled Water, 1ml
  • Control DNA, 20ml
 
*Tubes contain i-StarTaq DNA Polymerase, dNTPs, 10x Buffer, Mycoplasma primers, and 8-methoxypsoralen
 

Literature

 

Publications

· A Novel Small Molecule Activator of Nuclear Receptor SHP Inhibits HCC Cell Migration via Suppressing Ccl2

· Analysis of Transcriptional Variability in a Large Human iPSC Library Reveals Genetic and Non-genetic Determinants of Heterogeneity

· Chemistry-First Approach for Nomination of Personalized Treatment in Lung Cancer

· Computational discovery of pathway-level genetic vulnerabilities in non-small-cell lung cancer

· Defining Stage-Specific Activity of Potent New Inhibitors of Cryptosporidium parvum Growth In Vitro

· Inhibition of Nitric Oxide Production in Activated Macrophages Caused by Toxoplasma gondii Infection Occurs by Distinct Mechanisms in Different Mouse Macrophage Cell Lines

· Lactate Metabolism in Human Lung Tumors

· Monoclonal Antibodies to Intracellular Stages of Cryptosporidium parvum Define Life Cycle Progression In Vitro

· Multiscale Approaches to Complex Human Diseases

· p63 and SOX2 Dictate Glucose Reliance and Metabolic Vulnerabilities in Squamous Cell Carcinomas

· Selection at Work in Plasmodium Falciparum: Lessons From the Expanded Acyl CoA Synthetase Gene Family and in Vitro Artemisinin Resistance.

· Small cell lung cancer tumors and preclinical models display heterogeneity of neuroendocrine phenotypes

· Subtype-specific secretomic characterization of pulmonary neuroendocrine tumor cells

· Telomerase-Mediated Strategy for Overcoming Non–Small Cell Lung Cancer Targeted Therapy and Chemotherapy Resistance

· Therapeutic treatment of Marburg and Ravn virus infection in nonhuman primates with a human monoclonal antibody

· Toxoplasma Effector Recruits the Mi-2/NuRD Complex to Repress STAT1 Transcription and Block IFN-γ-Dependent Gene Expression