The ELISA-VIDITEST anti-VZV IgG kit is intended for
in vitro diagnosis of VZV (varicella-zoster virus)-associated diseases, namely varicella and herpes zoster. The test can also be utilized for the differential diagnosis of neuroinfections, infections of the eye and of exanthematous diseases of the skin. The measurements should be supplemented with the detection of IgM and IgA antibodies to VZV or with the determination of IgG avidity. Due to the q
uantitative evaluation, this kit can be used for determination of antibody concentration in International WHO-units (IU).
This assay is a solid-phase immuno-analytical test. The polystyrene strips are coated with a native antigen containing immunodominant epitopes of VZV. Anti-VZV antibodies from serum samples bind to the immobilized antigens. Antibodies that formed complexes with the antigens are later recognized by animal anti-human IgG antibodies labeled with horseradish peroxidase. The presence of the labeled antibodies is revealed by an enzymatic reaction with a chromogenic substrate.