The ELISA-VIDITEST anti-VZV IgM kit is intended for
in vitro diagnosis of VZV (varicella-zoster virus)-associated diseases, namely varicella and herpes zoster. The test can also be utilized for the differential diagnosis of neuroinfections, infections of the eye and of exanthematous diseases of the skin. The measurements should be supplemented with the detection of IgM and IgA antibodies to VZV or with the determination of IgG avidity. This kit contains RF sorbent for elimination of interfering IgG antibodies.
This assay is a solid-phase immunoanalytical test. The polystyrene strips are coated with a native antigen. Anti-VZV antibodies from serum samples bind to the immobilized antigens. Antibodies that formed complexes with the antigens are later recognized by animal anti-human IgG antibodies labeled with horseradish peroxidase. The presence of the labeled antibodies is revealed by an enzymatic reaction with a chromogenic substrate.