PCR PreMix (aliquoted & dried) Kits

Maximeâ„¢ RT-PCR PreMix Kit is a one-step RT-PCR product that synthesizes cDNA from RNA and amplifies the cDNA through PCR. This kit contains all components necessary for a RT-PCR reaction in a dried and stable format in each tube, including the high-performance OptiScriptâ„¢ RTase and the hot-start function i- StarTaqâ„¢ DNA Polymerase. RT-PCR can be carried out immediately with the addition of the RNA, gene-specific primer and distilled water, reducing the risk of pipetting error. The one-step RT-PCR method has the advantage of obtaining a higher specificity than the 2 steps method, making it ideal for the detection of trace amounts of infectious RNA in a sample. This kit has excellent stability due to oxidation and moisture prevention achieved by a vacuum compression manufacturing method.

Features

• Ready-to-use with all the reagents required for the reaction in a dried pellet in one tube
• RT-PCR can begin immediately by simply adding RNA and gene-specific primer 
• Gel loading electrophoresis is possible because tubes contain loading dye
• Excellent product stability and reproducibility
• Hot-start function enabled by i-StarTaqâ„¢ DNA Polymerase improves specificity and accuracy

Applications

• RNA qualification study
• RNA expression study
• Specific RNA detection
• Virus research
• Molecular diagnostic study

Contents

• Maximeâ„¢ RT-PCR PreMix, 96 tubes*

*Tubes include OptiScriptâ„¢ RT System, i-StarTaqâ„¢ DNA Polymerase, RT-PCR buffer(10x), stabilizer, and dNTP mixture