Maxime⢠RT-PCR PreMix Kit is a one-step RT-PCR product that synthesizes cDNA from RNA and amplifies the cDNA through PCR. This kit contains all components necessary for a RT-PCR reaction in a dried and stable format in each tube, including the high-performance OptiScript⢠RTase and the hot-start function i- StarTaq⢠DNA Polymerase. RT-PCR can be carried out immediately with the addition of the RNA, gene-specific primer and distilled water, reducing the risk of pipetting error. The one-step RT-PCR method has the advantage of obtaining a higher specificity than the 2 steps method, making it ideal for the detection of trace amounts of infectious RNA in a sample. This kit has excellent stability due to oxidation and moisture prevention achieved by a vacuum compression manufacturing method.
Features
- Ready-to-use with all the reagents required for the reaction in a dried pellet in one tube
- RT-PCR can begin immediately by simply adding RNA and gene-specific primer
- Gel loading electrophoresis is possible because tubes contain loading dye
- Excellent product stability and reproducibility
- Hot-start function enabled by i-StarTaq⢠DNA Polymerase improves specificity and accuracy
Applications
- RNA qualification study
- RNA expression study
- Specific RNA detection
- Virus research
- Molecular diagnostic study
Contents
- Maxime⢠RT-PCR PreMix, 96 tubes*
*Tubes include OptiScript⢠RT System, i-StarTaq⢠DNA Polymerase, RT-PCR buffer(10x), stabilizer, and dNTP mixture