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{{/_source.additionalInfo}}pORF-Clone Vector System
(Cat#: PORF)
Description
The yeast shuttle/expression vector pORF-CLONE has been specifically developed for improved and facilitated selection of cloned cDNA inserts containing open reading frames (ORFs). This allows an enriched growth of clones expressing authentic polypeptides.
This vector system is particularly useful for the development of a high-throughput technique for the one-step generation of high-quality cDNA libraries in the yeast Saccharomyces cerevisiae and for direct, time-saving screening of random-primed cDNA libraries.
The selection system is based on the HIS3 marker gene fused to the C-terminus of the cDNA insert. The cDNAs cloned in-frame result in histidine prototrophic yeast cells growing on minimal medium. Clones bearing the vector without insert or out of frame inserts should not grow on this medium.
- Improved cloning of open reading frames in yeast
- Efficient, cost-effective selection of cDNA clones with a correct pORF
- S. cerevisiae host systems allow expression of soluble, correctly folded and modified eukaryotic target proteins in high yields
- Improved selection of larger cDNA inserts in the eukaryotic host system, avoiding translation problems and frame-shifts
- Fast generation of high-quality cDNA libraries through random priming
- Easy, fast and reproducible purification of recombinant proteins via metal chelate affinity chromatography
- Detection of recombinant proteins in immunoblotting experiments by commercially available antibodies
- Ideally suited for high-throughput cloning or the production of cDNA chips
- pORF-CLONE vector DNA, 10 µg
- pORF-1 - PCR Primer, 500 pmole
- pORF-2 - PCR Primer, 500 pmole
Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
