i-pfu DNA Polymerase (High Fidelity)

Cat#: 25181
25181
Sales Price$195.00
Size
250 Units
Description

i-pfu DNA Polymerase is a typical heat-resistant proof-reading enzyme with 3'- 5' exonuclease activity. It has high fidelity and guarantees an error rate of 1x10-6 (one error rate per 13,000 base pairs). The proofreading activity of i-pfu DNA Polymerase results in PCR products with more accurate base sequences than when using Taq DNA Polymerase. When using i-pfu DNA Polymerase, DNA fragments longer than Taq DNA Polymerase (up to 30 kb) can be amplified. Due to these properties, i-pfu DNA Polymerase is widely used in experiments in which high accuracy is required, such as gene cloning and expression. Unlike Taq DNA Polymerase, this i-pfu DNA Polymerase produces blunt-end amplification products.

 

  • High accuracy makes it possible to amplify long DNA templates 
  • PCR products have more accurate bases 
  • Low error rate of 1x10-6
  • Used in blunt-end cloning
  • Ideal for experiments requiring high accuracy 
  • PCR
  • Blunt Cloning
  • Template amplification for DNA sequence analysis
  • i-pfu DNA Polymerase (2.5 U/ml), 250 units
  • 10x PCR Buffer (with 15 mM Mg2+), 1ml
  • 10x Mg2+-free PCR Buffer, 1ml
  • 10mM dNTPs (2.5 mM/each), 500μl 
  • 25mM Mg2+, 1ml

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