CRISPR Test Assays

Cellecta CRISPR-Test Assay Kits provide a convenient flow assay to accurately measure the activity of the Cas9 variant expressed by your cells. Each kit contains a mix of lentiviral particles with two different fluorescent markers. One fluorescent marker responds to the Cas9 or dCas9-variant activity measured by the kit, and the other marker provides a normalization control.

• Measure functional activity of Cas9 nuclease, dCas9-repressor, or dCas9-activator in any cell line
• Quantitative, flow-based, read-outs
• Convenient, complete, easy-to-use kits with ready-to-transduce lentiviral particles

Would you like to accurately measure the activity of CRISPR Cas9 nuclease (for CRISPR KO), dCas9-KRAB (for CRISPRi), or dCas9-activator such as dCas9-VPH (for CRISPRa) in your engineered cells before starting a CRISPR screen or other CRISPR study? Cellecta’s CRISPR-Test™ Assay Kits provide a convenient and quantitative assay for this purpose.

Each Assay Kit contains a mix of ready-to-transduce viral particles with one construct that is responsive to the Cas9 variant being measured, and the other, a negative control. Each of the constructs have different fluorescent protein markers (i.e., GFP or RFP) so the change in the level of one of the fluorescent markers relative to the other be assessed by flow analysis and provides an accurate measurement of the activity of the Cas9 variant expressed in the cells.

Additionally, a set of four Cas9 expression constructs is available at a reduced price when purchased in conjunction with one of the CRISPR-Test Assay Kits.

Example Citation:

• The chemotherapeutic CX-5461 primarily targets TOP2B and exhibits selective activity in high-risk neuroblastoma