Protein Markers and Ladders

The PM2800 ExcelBand 3-color Extra Range Protein Marker is a ready-to-use three-color protein standard with 13 pre-stained proteins covering an extra range of molecular weights from 10 to 310 kDa in Tris-Glycine buffer (9 to 290 kDa in Bis-Tris (MOPS) buffer and 10 to 290 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for three reference bands (one green and two red bands at 25 kDa and 75, 310 kDa respectively) when separated on SDS-PAGE in Tris-Glycine buffer. The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.

Features

• Ready-to-use, supplied in gel buffer
• Three-color protein standard with 13 pre-stained proteins
• Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25?), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol)

Applications

• Monitoring protein separation during SDS-polyacrylamide gel electrophoresis
• Verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon)
• Approximating size of proteins

Contents

• PM2800 ExcelBand 3-color Extra Range Protein Marker, 250 µl x 2