Anti-pIII (g3p) Antibody

Cat#: PSKAN3
antibody-symbol2
Sales Price$536.00
Size
100µl
Description

The monoclonal mouse anti P III (g3p) is an antibody to phage M13 coat protein pIII (g3p) monoclonal mouse IgG (Clone 10C3), Protein G purified. The antibody is supplied in PBS, pH 7.3 and supplemented with 0.2 mg/ml sodium azide. The anti-pIII (g3p) antibody is a useful analytical tool for phage display system using the coated protein III (g3-protein) for M13 for the display of recombinant proteins such as antibodies, protein – or peptide – libraries and more.  

The Epitope: the antigenic epitope of the anti-pIII (g3p) antibody resides within amino acids 292 to 302 of the mature protein III and is therefore compatible with most phagemid vectors using different N-terminal deletions of protein III.
 
  • Antibody to M13 coat protein III (g3p)
  • Clone: 10C3
  • Monoclonal Mouse IgG

This antibody can be used in Western blots to analyze:

  • Correct reading-frame fusions of pIII-fusion proteins
  • Expression levels of pIII fusion proteins in E. coli and on the tip of the phage
  • Ratios of displayed pIII-proteins and pIII-fusion proteins on the phage-capsid
  • M13-infected E. coli strains

The epitope is also recognized under non-denaturing conditions.  Therefore, the antibody can be used for:

  • ELISA detection of PIII-fusion proteins
  • Purification of pIII-fusion proteins by affinity chromatography
  • Efficient and rapid analysis of antigen binding characteristics of recombinant antibodies selected by phage display (microplate assay)
  • anti P III (g3p) - antibody, monoclonal mouse, 100 µl

Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.

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