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ReadyTector® All-in-One Solution for Western Blotting

730 000 - ReadyTector Anti-Rabbit-HRP All-in-One Detection Solution for Western Blotting

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Starting at: $108.00

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ReadyTector® - The All-in-One Solution for Western Blotting! 

ReadyTector® Anti-Rabbit-HRP contains ReadyTector® solution with Anti-Rabbit-HRP labeled secondary antibody and 10x wash buffer.
 
ReadyTector® includes everything you need for fast, all-in-one immunodetection. With one solution containing all components required, the entire process takes place in a single step, in addition to washing with the included wash buffer. Blocking and binding of the primary antibody, supplied by the user, and Anti-Rabbit-HRP labeled secondary antibody occur simultaneously. The ReadyTector® solution with the primary antibody can be re-used up to 5 times. Despite the fast, all-in-one incubation process, ReadyTector® reduces background, allowing users to generate clear, distinct bands suitable for publishing.
 
ReadyTector® is also available as Anti-Mouse-HRP. Additional bottles of 10x wash buffer can be purchased separately.
 
 
 
Figure 1: Protocol. Fast and easy with reduced background.
 
 
 
Figure 2: Results obtained with standard western blotting protocol and ReadyTector protocol. Lanes 1 – 5 contain 22, 11, 5.5, 2.8, 1.4ng. Alpha-1-Antitrypsin spiked in cell lysates, respectively. Protein detection: Mouse anti-A1AT Clone 1AT (Biotrend), 0.2 μg/mL on nitrocellulose. The primary antibody for A1AT should only show one band. Additional weak signals show non-specific extra bands.
 

Features

  • Easy one-step protocol with all-in-one solution
  • Fast procedure with quick results
  • Reduces background for clear, sharp bands
  • ReadyTector® solution with the primary antibody can be re-used up to 5 times
 

Applications

  • Western blotting
 

Sizes

 
730 040
  • ReadyTector® solution with Anti-Rabbit-HRP labeled secondary antibody, 40ml
  • 10x wash buffer, 40ml
730 120
  • ReadyTector® solution with Anti-Rabbit-HRP labeled secondary antibody, 120ml
  • 10x wash buffer, 120ml
730 500
  • ReadyTector® solution with Anti-Rabbit-HRP labeled secondary antibody, 500ml
  • 10x wash buffer, 500ml
 

Literature