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{{/_source.additionalInfo}}Broad-Host-Range Vector pBBR RESO (Promoter Trap Vector)
Cat#: RESO01
Description
pBBR RESO is a broad-host-range promoter cloning vector. It was derived from pBBR1MCS, which itself is a modification of the broad-host-range plasmid pBBR1CM. In contrast to other known broad-host-range vectors, pBBR RESO is maintained at a medium copy number and has a reasonable size of about 6.8 kb. It stably replicates in any Gram-negative bacterium studied and is therefore ideal for the isolation and genetic analysis of DNA sequences with promoter activity in the homologous organism. The reporter system used employs the resolvase-mediated excision of a kanamycin (Kan)-resistance gene flanked by two res sequences. Cloning an active promoter results in Kan-sensitive clones.
- Medium copy number and reasonable size
- Stably replicates in any Gram-negative bacterium
- Can identify genes under harsh environmental conditions
- Can detect both constitutively and transiently induced promoters
- Identification of promoter sequence of a cloned gene or operon
- Identification of promoter sequences in random tnpR-gene fusion libraries
- Identification of genes induced under harsh environmental conditions
- pBBR RESO vector, lyophilized DNA, 5 µg
Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
