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{{/_source.additionalInfo}}G-HiFi DNA Polymerase
Cat#: TF3000
Description
G-HiFi DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for GC-rich templates that are difficult to amplify.
The fidelity of G-HiFi DNA Polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of G-HiFi DNA Polymerase is achieved by blending the DNA polymerase with an elongation enhancer.
The optimized 5X G-HiFi Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized 5X G-HiFi Buffer, G-HiFi DNA Polymerase is capable to amplify most templates, such as longer targets (up to 40 kb from lambda DNA) and targets that contain GC-rich sequences.
The storage buffer is composed of 50 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol.
- 5’→3’ DNA polymerase activity
- 3’→5’ exonuclease (proofreading) activity
- High reaction rate = 7 seconds/kb
- High fidelity = 70 times higher than Taq polymerase
- Generates blunt end amplicons
- Vast elongation capability (up to 40 kb)
- Thermo-stable for more than 10 hours at 95°C
- Long range PCR amplification
- PCR for DNA sequencing
- Amplification of GC-rich templates
- G-HiFi DNA Polymerase, 100 μl
- 5X G-HiFi Buffer, 1200 μl
- dNTPs Mix (2 mM each), 600 μl
Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
