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RmesFect mRNA Transfection

RM20000 - RmesFect™-mRNA transfection

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Starting at: $250.00

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RmesFect™ Transfection Reagent is specifically designed for in vitro mRNA transfection with high efficiency and low toxicity.
 
Unlike plasmid DNA delivery, mRNA transfection is advantageous in that it does not require nuclear uptake and it is not integrative. RmesFect™ is based on TEE technology (Triggered Endosomal Escape) that combines and exploits the properties of cationic lipids and polymers. The cationic design of RmesFect™ protects mRNA from degradation for efficient transport directly into the cytosol. 
 
Figure 1: Several cell lines were transfected with 0.5 μg of mRNA encoding GFP protein and RmesFect™ transfection reagent (ratio 2:1 for RAW264.7,3:1 for COS7 and CHO-K1 and 4:1 for HeLa cells). After 20 min of incubation at room temperature, complexes were added onto the cells in a dropwise manner. 24 H after, transfection efficiency was measured by FACS analysis.
 
 
 
Figure 2: Several cell lines were transfected with  mRNA encoding GFP protein and RmesFect™ transfection reagent. 24 H after, transfection efficiency was measured by fluorescence microscopy.
 

Features

  • Specifically designed for mRNA transfection
  • High efficiency and low toxicity
  • Can be used with all types of cells
  • Protects mRNA against degradation
  • Compatible with serum and any culture medium
 

Applications

  • Transfection of mRNA in primary cells and cell lines
  • mRNA vaccines, primary cells transfection, regenerative medicine, cell reprogramming, iPs generation, etc.
 

Sizes

  • RM20500 - RmesFect™ Transfection Reagent, 500 µl (125-250 transfections)
  • RM21000 - RmesFect™ Transfection Reagent, 1ml (250-500 transfections)
  • RM25000 - RmesFect™ Transfection Reagent, 5ml (1250-2500 transfections)