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Human Antibodies

151784 - Anti-BCLAF1 [M33-P5B11]


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Cat# 151874
Applications ELISA, IHC, WB
Antigen/Gene or Protein Targets BCL2 Associated Transcription Factor 1 (BCLAF1)
Reactivity Human
Relevance BCLAF1 was associated with poor prognosis and showed aberrant localization in colorectal cancer.
Host Mouse
Immunogen Peptide Sequence - KYQGDGIVED (amino acids 900 – 909)
Positive Control ELISA- Peptide immunogenWestern Blot- Hela whole cell extractIHC- Human colon carcinoma
Subclass IgG1 kappa
Recommended Growing Conditions Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM + 60 ml Fetal Bovine Serum plus 3ml L-Glutamine(Sigma G7513) + 3ml of Penicillin -Streptomycin (Sigma P0781).
Strain Balb/c
Research Area Apoptosis and Programmed Cell Death, Cancer, Epigenetics & Nuclear Signalling
References The expression and prognostic significance of bcl-2-associated transcription factor 1 in rectal cancer following neoadjuvant therapy. Europe PMC ID: 26183150


Image: Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections using Anti-BCLAF1 [M33-P5B11]. Image is showing both cytoplasmic and nuclear localisation positivity of BCLAF1 in colon cancer which is in agreement with existing literature. Exclusively nuclear localisation of BCLAF1 is observed in non-neoplastic colon mucosa. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).


For Research Use Only