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Human Antibodies

151836 - Anti-TIMP1 [2A5]


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Cat# 151836
Applications ELISA, IHC, WB
Antigen/Gene or Protein Targets TIMP1 (tissue inhibitor of metalloproteinases 1)
Reactivity Human
Relevance Matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes as well as in disease processes. Tissue inhibitors of metalloproteinases (TIMPs) are the main physiological regulators of the MMPs. The TIMPs are secreted proteins that complex with individual MMPs and regulate the activity of specific MMPs. Together, the MMPs and TIMPs form a complex biological system strictly controlling degradation of ECM. The MMPs and TIMPs have a significant role in facilitating tumor invasion and metastasis.

Expression of TIMP1 has been identified in individual studies as prognostic biomarkers in established and locally advanced colorectal cancer.
Host Mouse
Immunogen Ovalbumin-conjugated synthetic peptide; FQALGDAADIR
Positive Control IHC: formalin-fixed, paraffin-embedded colonic adenocarcinoma. Western blot: rhTIMP-1, 400 ng per lane
Subclass IgG1 kappa
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions Dulbecco’s media containing L-Glutamine and 20% Fetal Bovine serum (DH20).
Strain Balb/c
Notes IHC: A recommended dilution of 1/8. Recommended pretreatment of antigen retrieval: microwave for 7 minutes at 950W in 0.01M sodium citrate buffer, pH 6.0.

Western blot: not reactive with TIMP-2. A recommended dilution of 1/5.
Research Area Adhesion, Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Protein Degradation
References The matrix metalloproteinase/tissue inhibitor of matrix metalloproteinase profile in colorectal polyp cancers. Europe PMC ID: 19635101

Profiling markers of prognosis in colorectal cancer. Europe PMC ID: 16489072

Matrix metalloproteinase/tissue inhibitors of matrix metalloproteinase phenotype identifies poor prognosis colorectal cancers. Europe PMC ID: 15623598

Matrix metalloproteinases and their inhibitors in gastric cancer. Europe PMC ID: 9824606


For Research Use Only