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Pathogen Antibodies

153344 - Anti-PKD [P14G8]

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Cat# 153344
Applications ELISA, IHC, WB
Antigen/Gene or Protein Targets Tetracapsuloides bryosalmonae parasite antigen P14G8
Synonyms P14G8; T. bryosalmonae P14G8
Reactivity Tetracapsuloides bryosalmonae
Relevance The myxozoan parasite Tetracapsuloides bryosalmonae infects salmonid fishes. It causes proliferative kidney disease (PKD), one of the most serious parasitic diseases of salmonid populations in Europe and North America, which causes losses of up to 90% in infected populations. [P14G8] targets Tetracapsuloides bryosalmonae P14G8 antigen and is species-specific. The mAb detects the extrasporogonic stage of the parasite.
Host Mouse
Immunogen Peptide Sequence specific to P14G8 parasite antigen
Positive Control Western Blot: Infected fish kidney cells. IHC: formalin-fixed, paraffin-embedded multi tumor tissue microarray (kidney from healthy fish versus kidney from infected fish).
Subclass IgG
Myeloma Used P3X63Ag8.653
Strain Balb/c
Notes This antibody is potentially neutralizing and can be used for diagnostics and to study parasite-host interactions. Recommended Usage Conditions: ELISA neat; Western Blot neat; IHC neat (antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0).
Research Area Aquaculture, Parasitology

 

Image: Immunohistochemistry images are showing staining in (A) uninfected kidney tissue and (B) infected kidney using Anti-PKD [P14G8]. Arrows indicate parasite.

 

For Research Use Only.