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{{/_source.additionalInfo}}Xpert Fast HRM (2x MasterMix)
1ml (Cat# GE70.0100)
Description
High Resolution Melting (HRM) analysis presents a powerful tool for detecting mutations and polymorphisms in dsDNA samples, including CpG methylation differences. At increasing temperature, in the presence of a fluorescent dye, minute sequence variations between samples result in different DNA strand dissociation behavior. HRM analysis exploits these differences using the melting curve shapes to distinguish between the samples.
Xpert Fast HRM (2x Mastermix) contains all of the components except the specific primers and template, for HRM analysis in qPCR. The mix consists of the combination of a highly efficient enzyme with a highly optimized buffer system, which allows for the efficient amplification of GC-rich and AT-rich templates under both normal and fast PCR conditions. The intercalating dye in the mastermix does not cause PCR inhibition, therefore allowing for extremely high sensitivity and specificity, as well as preventing the formation of unwanted primer-dimers and non-specific products.
Different SNPs show differing Tm melt curve shifts. Class I SNPs (C/T and G/A) demonstrate typically large shifts (>.5C), whereas Class IV SNP (A/T) typically have much smaller shifts (<.2C). The high sensitivity and specificity of Xpert Fast HRM allows the user to distinguish all possible SNPs.
Storage is -20C for 1 year.
Contains:
- 1ml of Mastermix (2x), containing an optimized ration of fast DNA polymerase, dNTPs, MgCl2, and non-PCR inhibiting intercalating green dye
- Sufficient for 100 rxns of 20ul
- Excellent signal and low PCR inhibition
- Ultra-sensitive melting profiles
- Superior amplification from GC and AT-rich sequences
- Allows for both standard and fast cycling
- Contains a next generation intercalating dye
- Extreme specificity
- High Resolution Melting (HRM) Analysis
- SNP Genotyping
- CpG methylation analysis (by comparing bisulfite-treated and control DNA)
GE70.0100 (1ml)
GE70.5100 (5 x 1ml)
GE70.25010 (25 x 1ml)
Related Categories
DNA Methylation KitsBoca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
