One Step Whole Genome Amplification Kit

50 Reactions (Cat# 108750)
wgakit
Sales Price$701.00
Size 
Description

The One Step Whole Genome Amplification Kit is an improved version of the DOP (degenerated oligo PCR) with optimized primer sequences and our unique SD Polymerase. This enzyme combines the strand displacement capability with heat stability up to 92°C. Due to these features, it is possible to use this enzyme for both amplification steps without additional pipetting or handling.

The WGA Kit is designed for reproducible amplification of low copy number genomic DNA to yield 2-10 µg of the product in about 2 hours. All components necessary for amplification of human genomic DNA from single cells or low copy templates are included.

For applications that require cell lysis, an improved version of One Step WGA Kit, the Single Cell WGA Kit, should be used. 

 
  • Amplification finished in less than 2 hours
  • Different genomic amplification coverage than other enzymes
  • Long fragments up to 3 Kb
  • One Enzyme for the whole genome amplification
  • Ready for PCR and NGS
  • Suitable for single cell DNA Analysis
  • Single cell DNA analysis
  • Next Generation Sequencing (NGS)
  • SNP Genotyping
  • qPCR- and PCR-based mutation detection
  • STR and RFLP analysis
  • Array technologies (comparative genomic hybridization)
  • 108725 - 25 reactions
  • 108750 - 50 reactions
Kit Contents:
  • 2.5x Master Mix without Enzyme
  • SD Polymerase, 10 U/µl
  • PCR grade H2O
  • Human genomic DNA as positive control

Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.

Recently Viewed Products