SD Polymerase

Unique, thermostable polymerase for isothermal amplification and PCR.

SD Polymerase is a novel, artificial polymerase with a strong strand displacement activity and heat stability up to 93°C,  but lacking 5′-3′ exonuclease activity. With SD Polymerase, the possibilities of isothermal amplification and regular PCR can be combined. 

Natural enzymes with a strong strand displacement activity, such as Phi29 or Bst Polymerase, are active below 68°C only. With the high heat stability of SD Polymerase, the efficiency of isothermal amplification can be enhanced by initial denaturation steps to increase the accessibility of single strands. The initial heating-up improves the specificity in applications such as LAMP, library generation, PCDR, WGA and standard PCR. 

SD Polymerase is suitable for high GC-content and difficult targets. Long Range PCR can be performed with target sizes up to 30 kb. 

This enzyme has 5'-3' polymerase and 5'-3' strand displacement activities, as well as 3'-A-overhangs. It does not have any exonuclease activity. 

Multiple formats are available, each of which includes a reaction buffer and MgCl₂:

• 10 U/µl 
• High concentration, 50 U/µl
• HotStart, 10 U/µl 

SD Polymerase Hotstart 10 U/µl is a novel artificial thermostable polymerase with strong Strand Displacement, but lacking 5′-3′ exonuclease activity. The efficiency of Isothermal Amplification can be raised by initial denaturation steps to increase the accessibility of single strands. SD Polymerase Hotstart is efficient for high GC-content and difficult targets. Long Range PCR can be performed with target size up to 30 kb. The optimal blocking by antibody inactivates the enzyme until initial heating. SD Polymerase Hotstart can be used in various strand displacement reactions like WGA, Isothermal Amplification, PCDR, library generation and standard PCR reactions. For LAMP assays we strongly recommend SD Polymerase 50 U/µl without antibody.