Search
Search
{{#data.error.root_cause}}
{{/data.error}}
{{^data.error}}
{{#texts.summary}}
[{{{type}}}] {{{reason}}}
{{/data.error.root_cause}}{{texts.summary}} {{#options.result.rssIcon}} RSS {{/options.result.rssIcon}}
{{/texts.summary}} {{#data.hits.hits}}
{{#_source.featured}}
FEATURED
{{/_source.featured}}
{{#_source.showImage}}
{{#_source.image}}
{{/_source.image}}
{{/_source.showImage}}
{{/data.hits.hits}}
{{{_source.title}}} {{#_source.showPrice}} {{{_source.displayPrice}}} {{/_source.showPrice}}
{{#_source.showLink}} {{/_source.showLink}} {{#_source.showDate}}{{{_source.displayDate}}}
{{/_source.showDate}}{{{_source.description}}}
{{#_source.additionalInfo}}{{#_source.additionalFields}} {{#title}} {{{label}}}: {{{title}}} {{/title}} {{/_source.additionalFields}}
{{/_source.additionalInfo}}DNA Fragmentation Kit
50 Preps (Cat# 131050)
Description
Bioron's DNA Fragmentation Kits is designed for enzymatic fragmentation of genomic DNA (100-1000 ng) for preparation of a DNA library for Next Generation Sequencing. The length of generated double-stranded (ds) DNA fragments can be easily adjusted from 100 to 800 bp based on the concentration of magnesium chloride.
The enzymatic fragmentation method used in the kit is based on a combination of two enzymes: nonspecific endonuclease DNase I and Bioron's unique SD Polymerase, a recombinant Taq DNA polymerase with strand displacement activity. This enzyme is stable at temperatures up to 93°C and has a maximum level of enzymatic activity at 70-75°C.
At the first stage, DNase I introduces many random single-strand breaks (nicks) into dsDNA. At the second stage, with an increase in the temperature of the mixture, endonuclease DNase I is inactivated and SD Polymerase is activated. Many overlapping elongated double-stranded fragments with 3'-A-overhangs at the ends are generated. The fragments are suitable for direct ligation with DNA adapters, bypassing the additional steps of repairing the ends of DNA fragments and adenylation.
- Enzymatic fragmentation based on combination of nonspecific endonuclease DNase I and SD Polymerase
- Fragments are suitable for direct ligation with DNA adapters
- Generated fragments are 100-800 bp
- Preparation of DNA library for Next Generation Sequencing
- 131025 - 25 preps
- 131050 - 50 preps
Kit Contents:
- Reaction Buffer
- Enzyme Mix
- MgCl2
- Water
- Control DNA
- Magnetic Beads
Related Products
SD PolymeraseBoca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
