Description
Sensitivity and specificity of antibody detection assays for COVID-19 continue to present challenges. This sampler package contains all the reagents you need for serological ELISA, complete with blockers, stabilizers and optimizers. These reagents will greatly improve the accuracy of your assay and significantly reduce false positives and false negatives.
125ml of each reagent is included:
- PlateBlock™ is an animal and protein-free blocker specifically designed for serological assays (antigen-down assays) on plastic surfaces. It successfully reduces false positives and improves the assay reliability.
- LowCross-Buffer® STRONG is an innovative assay buffer that greatly improves the quality of detection by reducing immunoassay interference such as nonspecific binding, cross-reactivities and matrix effects. This reagent is an alternate version of LowCross-Buffer®. With LowCross-Buffer® STRONG, the differentiation-line between false and true signals has been moved to higher affinities compared to LowCross-Buffer®.
- Liquid Plate Sealer® Animal-Free is a stabilizer for coated antibodies and antigens on polystyrene or glass surfaces. Treated plates can be stored for long periods after drying. This reagent is an alternate version of the standard Liquid Plate Sealer® made without any animal derived ingrediants.
- HRP-Protector™ is a long-term stabilizer for horseradish peroxidase (HRP) coupled to antibodies or Neutravidin/Streptavidin. It can be used directly as an assay buffer for incubating the HRP-conjugates.
- Washing Buffer (10x concentrate) is used in immunoassays to remove interfering unbound and excessive components. The version included in this package is based on TRIS, but it is also available based on PBS and without tween for IHC.
- Coating Buffer (pH 7.4) is designed for adsorptive immobilization of proteins and antibodies on plastic surfaces, such as microtiter plates, or other protein binding surfaces. The pH 7.4 version is based on PBS.
A detailed protocol is available for detection of IgG antibodies against the RBD of SARS-CoV-2. This protocol can be modified for other serological assays as well. Learn more
With the innovative reagents in this sampler package, reliable results in antibody testing can be obtained.
Reagent |
Quantity |
Datasheet |
PlateBlock™ |
125ml |
Pdf |
LowCross-Buffer® STRONG |
125ml |
Pdf |
Liquid Plate Sealer® Animal-Free |
125ml |
Pdf |
HRP-Protector™ |
125ml |
Pdf |
Washing Buffer (10x Concentrate) Based on TRIS |
125ml |
Pdf |
Coating Buffer (pH 7.4) |
125ml |
Pdf |