The Bacillus subtilis system was developed as a host for gram-positive bacteria in a variety of applications, including agricultural, medical and food biotechnology and the production of recombinant proteins.
The Bacillus subtilis system is advantageous in that it is non-pathogenic, does not have significant bias in codon usage, and is capable of secreting functional extracellular proteins directly into the culture medium. A large body of information concerning transcription, translation, protein folding and secretion mechanisms, genetic manipulation, and large-scale fermentation is available.
The B. subtilis Pgrac01 expression system provides an easy-to-handle tool for high yield intracellular protein production with B. subtilis and for secretion of proteins into the culture medium in large amounts. Expression of the gene of interest is regulated by the Pgrac01 promoter fused to the lac operator, allowing the induction by addition of IPTG.
Bacillus subtilis control vectors are also available.
Please note these products require a license for commercial companies. Please contact us for licensing information.
|PBS001||pHT01||All subsequent vectors are derivatives of pHT01|
|PBS002||pHT43 (secretion)||amyQ signal sequence for protein export|
|PBS003||pHT08||C-terminal 8xHis tag|
|PBS004||pHT09||C-terminal Strep tag|
|PBS005||pHT10||C-terminal c-Myc tag|
|PBS010||pHT1464 (secretion)||Improved amyQ signal sequence for extended protein export|
All vectors are supplied as lyophilized DNA (10µg).
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